Expression of endoglucanases in Pichia pastoris under control of the GAP promoter

@inproceedings{Vrnai2014ExpressionOE,
  title={Expression of endoglucanases in Pichia pastoris under control of the GAP promoter},
  author={Anik{\'o} V{\'a}rnai and Campbell Tang and Oskar Bengtsson and Andrew Atterton and Geir Mathiesen and Vincent G. H. Eijsink},
  booktitle={Microbial cell factories},
  year={2014}
}
BACKGROUND Plant-derived biomass is a potential alternative to fossil feedstocks for a greener economy. Enzymatic saccharification of biomass has been studied extensively and endoglucanases have been found to be a prerequisite for quick initial liquefaction of biomass under industrial conditions. Pichia pastoris, widely used for heterologous protein expression, can be utilized for fungal endoglucanase production. The recently marketed PichiaPink™ expression system allows for rapid clone… CONTINUE READING
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