Expression of distinct RNAs from 3′ untranslated regions
@article{Mercer2011ExpressionOD, title={Expression of distinct RNAs from 3′ untranslated regions}, author={Tim R. Mercer and Dagmar Wilhelm and M. Dinger and Giulia Sold{\`a} and Darren Korbie and Evgeny A. Glazov and Vy Truong and Maren Schwenke and Cas Simons and Klaus Ingo Matthaei and Robert B. Saint and Peter Koopman and John S. A. Mattick}, journal={Nucleic Acids Research}, year={2011}, volume={39}, pages={2393 - 2403} }
The 3′ untranslated regions (3′UTRs) of eukaryotic genes regulate mRNA stability, localization and translation. Here, we present evidence that large numbers of 3′UTRs in human, mouse and fly are also expressed separately from the associated protein-coding sequences to which they are normally linked, likely by post-transcriptional cleavage. Analysis of CAGE (capped analysis of gene expression), SAGE (serial analysis of gene expression) and cDNA libraries, as well as microarray expression…
192 Citations
Terminus-Associated Non-coding RNAs: Trash or Treasure?
- BiologyFrontiers in Genetics
- 2020
Current knowledge of the potential role of TANRs in the modulation of gene expression is reviewed, and TANR nomenclature, relation to ncRNAs, cross-talk biogenesis pathways and potential functions are discussed.
Regulation by 3'-Untranslated Regions.
- BiologyAnnual review of genetics
- 2017
3'-UTRs seem to be major players in gene regulation that enable local functions, compartmentalization, and cooperativity, which makes them important tools for the regulation of phenotypic diversity of higher organisms.
Emerging Roles for 3′ UTRs in Neurons
- BiologyInternational journal of molecular sciences
- 2020
Surprising roles for 3′ UTRs are emerging beyond the regulation of protein synthesis and include roles as RBP delivery scaffolds and regulators of alternative splicing.
Detection of Differentially Expressed Cleavage Site Intervals Within 3′ Untranslated Regions Using CSI-UTR Reveals Regulated Interaction Motifs
- BiologyFront. Genet.
- 2019
This work developed a method, CSI-UTR, that models 3′UTR structure as tandem segments between functional alternative-polyadenylation sites (termed cleavage site intervals—CSIs) and identified a set of CSIs in human and rodent transcriptomes.
Cytoplasmic cleavage of IMPA1 3′ UTR is necessary for maintaining axon integrity
- BiologyCell reports
- 2021
Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae
- BiologyRussian Journal of Genetics
- 2014
Yeast might be used as an appropriate model system for studies of 3′-UTR’s role in post-transcriptional regulation and the potential sites of yeast transcriptional termination in Drosophila limk1 gene encoding for the key enzyme of actin remodeling.
Regulation of eukaryotic gene expression by the untranslated gene regions and other non-coding elements
- BiologyCellular and Molecular Life Sciences
- 2012
Recent insights into the regulatory roles of the untranslated gene regions and non-coding RNAs in the control of complex gene expression are discussed, as well as the implications of this in terms of organism complexity and evolution.
Post-transcriptional 3´-UTR cleavage of mRNA transcripts generates thousands of stable uncapped autonomous RNA fragments
- BiologyNature Communications
- 2017
Evidence that mRNA can be cleaved post-transcriptionally to generate mRNAs with shorter 3-´UTRs and stable autonomous uncapped 3´-UTR sequences is provided.
3’UTR cleavage of transcripts localized in axons of sympathetic neurons
- Biology
- 2017
Analysis of the Inositol monophosphatase 1 (Impa1) mRNA revealed that a stem loop structure within the 3’UTR is necessary for Ago2 cleavage, which provides an alternative mechanism that simultaneously regulates local protein synthesis and generates a new class of 3'UTR RNAs with yet unknown functions.
Independent 3’untranslated region RNA, a novel non-coding regulator RNA: what do we know now?
- Biology
- 2014
Evidence has been presented that the I3’UTRs generally exist in eukaryotes, and are a novel regulating RNA species that is worth of thorough and in-depth investigations.
References
SHOWING 1-10 OF 57 REFERENCES
Proliferating Cells Express mRNAs with Shortened 3' Untranslated Regions and Fewer MicroRNA Target Sites
- BiologyScience
- 2008
It is found that states of increased proliferation are associated with widespread reductions in the 3′UTR-based regulatory capacity of mRNAs, which is a characteristic of gene expression during immune cell activation and correlates with proliferation across diverse cell types and tissues.
Post-transcriptional processing generates a diversity of 5′-modified long and short RNAs
- BiologyNature
- 2009
It is shown that processing of mature mRNAs through an as yet unknown mechanism may generate complex populations of both long and short RNAs whose apparently capped 5′ ends coincide.
Translational control by the 3'-UTR: the ends specify the means.
- BiologyTrends in biochemical sciences
- 2003
Specific expression of long noncoding RNAs in the mouse brain
- BiologyProceedings of the National Academy of Sciences
- 2008
The results show that the majority of ncRNAs are expressed in the brain and provide strong evidence that themajority of processed transcripts with no protein-coding capacity function intrinsically as RNAs.
The Transcriptional Landscape of the Mammalian Genome
- BiologyScience
- 2005
Detailed polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development.
Regulated post-transcriptional RNA cleavage diversifies the eukaryotic transcriptome.
- BiologyGenome research
- 2010
It is shown that post-transcriptional cleavage of RNA contributes to the diversification of the transcriptome by generating a range of small RNAs and long coding and noncoding RNAs that retain the potential to be translated into shortened functional protein isoforms.
Genetic complementation reveals a novel regulatory role for 3′ untranslated regions in growth and differentiation
- BiologyCell
- 1993
Diverse endonucleolytic cleavage sites in the mammalian transcriptome depend upon microRNAs, Drosha, and additional nucleases.
- BiologyMolecular cell
- 2010
Tumor suppression by RNA from the 3' untranslated region of alpha-tropomyosin.
- BiologyCell
- 1993
It is concluded that untranslated RNAs can function as regulators (riboregulators) that suppress tumor formation in NMU2 cells and that suppression of tumorigenicity is dependent on the continued expression of Tm transcripts lacking a coding region.