Expression of dbat and dbtnbt genes involved in paclitaxel biosynthesis during the growth cycle of Taxus baccata L. callus cultures.

@article{Katkovinov2008ExpressionOD,
  title={Expression of dbat and dbtnbt genes involved in paclitaxel biosynthesis during the growth cycle of Taxus baccata L. callus cultures.},
  author={Zuzana Katkov{\vc}inov{\'a} and Martina L{\'a}z{\'a}rov{\'a} and Katar{\'i}na Bruň{\'a}kov{\'a} and J{\'a}n Ko{\vs}uth and Eva {\vC}ell{\'a}rov{\'a}},
  journal={Zeitschrift fur Naturforschung. C, Journal of biosciences},
  year={2008},
  volume={63 9-10},
  pages={721-30}
}
The time-course of expression of dbat and dbtnbt genes involved in the later steps of paclitaxel biosynthesis and the intracellular taxane accumulation were investigated through a 64-day subculture interval of VI/M1 and VI/M2 Taxus baccata callus cultures. HPLC proved traces of baccatin III and an intracellular content of paclitaxel up to 90 microg/g DW. The steady-state of the respective gene transcripts was measured by quantitative real-time RT-PCR. The expression profile of dbat and dbtnbt… CONTINUE READING