Expression of cytosolic phospholipase A2 alpha in murine C12 cells, a variant of L929 cells, induces arachidonic acid release in response to phorbol myristate acetate and Ca2+ ionophores, but not to tumor necrosis factor-alpha.

@article{Shimizu2004ExpressionOC,
  title={Expression of cytosolic phospholipase A2 alpha in murine C12 cells, a variant of L929 cells, induces arachidonic acid release in response to phorbol myristate acetate and Ca2+ ionophores, but not to tumor necrosis factor-alpha.},
  author={Masaya Shimizu and Chihiro Azuma and Tomoko Taniguchi and Toshihiko Murayama},
  journal={Journal of pharmacological sciences},
  year={2004},
  volume={96 3},
  pages={
          324-32
        }
}
Tumor necrosis factor-alpha (TNFalpha)-induced cell death is regulated through the release of arachidonic acid (AA) by group IVA cytosolic phospholipase A2 (cPLA2alpha) in the murine fibroblast cell line L929. However, the signaling pathway by which TNFalpha activates cPLA2alpha remained to be solved. We examined AA release in L929 cells, in a variant of L929 (C12 cells) lacking cPLA2alpha, and in C12 cells transfected with cPLA2alpha expression vectors. In transient and stable clones of C12… Expand
Vanadate-induced activation of cytosolic phospholipase A2alpha in L929 cells: Roles of tyrosine kinase, protein kinase C, and extracellular signal-regulated kinase.
TLDR
Roles of alpha-type cytosolic phospholipase A2 (cPLA2alpha), Src family kinases (Src) and protein kinase C (PKC) in the release of AA induced by Na3VO4 from a murine fibroblast cell line, L929, are investigated. Expand
Hydrogen peroxide-induced arachidonic acid release in L929 cells; roles of Src, protein kinase C and cytosolic phospholipase A2α
TLDR
It is suggested that H 2 O 2 with Ca 2+ stimulates arachidonic acid release via cPLA 2 α in a Src- and PKC-dependent manner in L929 cells. Expand
Release of arachidonic acid induced by tumor necrosis factor-alpha in the presence of caspase inhibition: evidence for a cytosolic phospholipase A2alpha-independent pathway.
TLDR
TNFalpha/zVAD appears to stimulate release of AA from C12 cells in a cPLA(2)alpha-independent, BHA-sensitive manner and reactive oxygen species from different pools in the release ofAA and cell death were discussed. Expand
Activation of cytosolic phospholipase A2alpha by epidermal growth factor (EGF) and phorbol ester in HeLa cells: different effects of inhibitors for EGF receptor, protein kinase C, Src, and C-Raf.
TLDR
Parmacological experiments showed that the responses (ERK phosphorylation and AA release) induced by EGF and PMA were mediated by a mitogen-activated protein kinase/ERK kinase (MEK)-ERK-alpha-type cytosolic phospholipase A(2) (cPLA( 2)alpha) pathway and that EGFR couples with the pathway in a manner insensitive to sorafenib. Expand
Release of arachidonic acid by 2-arachidonoyl glycerol and HU210 in PC12 cells; roles of Src, phospholipase C and cytosolic phospholipase A(2)alpha.
TLDR
Analysis of signaling mechanism for the release of arachidonic acid by cannabinoids and selective inhibitors reveals that cannabinoids activated cPLA(2)alpha in a Src-phospholipase C-protein kinaseC-dependent manner probably via cannabinoid CB(1) receptor and/orCB(1)-like receptor in neuronal cells. Expand
Amyloid beta enhances cytosolic phospholipase A2 level and arachidonic acid release via nitric oxide in APP-transfected PC12 cells.
TLDR
Results indicate that Abeta peptides enhance the protein level and phosphorylation of cPLA2 and AA release by the NO signaling pathway. Expand
Possible Involvement of Intracellular Calcium-Independent Phospholipase A2 in the Release of Secretory Phospholipases from Mast Cells—Increased Expression in Ileal Mast Cells of Crohn’s Disease
TLDR
It is suggested that iPLA2β-mediated release of sPLA2 from intestinal MCs may contribute to CD pathophysiology and ex vivo studies on isolated mucosal mast cells are needed to clarify the precise role of MC PLA2s in the inflammatory processes of CD. Expand
Ser515 phosphorylation-independent regulation of cytosolic phospholipase A2alpha (cPLA2alpha) by calmodulin-dependent protein kinase: possible interaction with catalytic domain A of cPLA2alpha.
TLDR
Findings show a possible effect of CaM kinase on cPLA(2)alpha in a catalytic domain A-dependent and Ser515-independent manner. Expand
Inhibition of cytosolic Phospholipase A2 prevents prion peptide-induced neuronal damage and co-localisation with Beta III Tubulin
TLDR
It is suggested that cPLA2 plays a vital role in the action of HuPrP106-126 and that the colocalisation of p-cPLA2 with beta III tubulin could be central to the progress of neurodegeneration caused by prion peptides. Expand
Effects of synthetic sphingosine-1-phosphate analogs on cytosolic phospholipase A2alpha-independent release of arachidonic acid and cell toxicity in L929 fibrosarcoma cells: the structure-activity relationship.
TLDR
Synthetic phosphorylated lipid analogs may be useful for studying PLA(2) activity and its toxicity in cells, and a high correlation coefficient was observed. Expand
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