Expression of corticosteroid-binding globulin mRNA in human uterine endometrial cancers

  title={Expression of corticosteroid-binding globulin mRNA in human uterine endometrial cancers},
  author={Ryou Misao and Yoshihito Nakanishi and Jiro Fujimoto and Satoshi Ichigo and Masashi Hori and Teruhiko Tamaya},
Since it has been demonstrated that corticosteroid-binding globulin (CBG) plays a role in intracellar steroidal actions in target cells, the expression of CBG mRNA as the measure of CBG expression was investigated in human endometrial cancers in order to assess the biological implications of CBG. The level of CBG mRNA was analyzed using competitive reverse transcription-polymerase chain reaction-Southern blot analysis. While the level of CBG mRNA was significantly (P < 0.01) higher in secretory… Expand
Effects of Sex Steroid Hormones on Corticosteroid-Binding Globulin Gene Expression in Human Endometrial Cancer Cell Line Ishikawa
Findings suggest that the effects of high-dose progestins on cancer cells may be mediated via suppression of intracellular CBG. Expand
Evidence for the Synthesis of Corticosteroid-Binding Globulin in Human Placenta
Findings suggest that CBG is synthesized in human placenta during pregnancy in addition to its synthesis in the liver. Expand
Expression of oestrogen receptor α and β mRNA in corpus luteum of human subjects
This study demonstrates that ERb is co-expressed with ERa in human corpus luteum and is likely to play a biological role in the regulation of steroidal action of the corpus lutesum with ERb. Expand
Levels of sex hormone-binding globulin and corticosteroid-binding globulin mRNAs in corpus luteum of human subjects: correlation with serum steroid hormone levels.
The findings suggest that the synthesis of luteal SHBG and CBG is complexly regulated by estrogen and progesterone, and that SHBGand CBG interact with estrogen andprogesterone, respectively, for lutenal steroidal activity. Expand
Steroid-binding proteins and free steroids in birds
Evidence for the biological relevance of each fraction of glucocorticoid hormone; the CBG-glucocortioid complex (the bound fraction) and the remainder which is either unbound or loosely attached to albumin (the free fraction) is reviewed. Expand
Plasma binding proteins as mediators of corticosteroid action in vertebrates.
It is proposed that consideration of CBG is paramount to understanding the role of glucocorticoids in mediating behavioral and physiological responses to stress. Expand
Identification of Exon-Deleted Progesterone Receptor mRNAs in Human Uterine Endometrial Cancers
All translated variant proteins might possess functional diversity and might modify the progestational action of wild-type PR, and the expression of some PR variant mRNAs may be lost as endometrial cancer cells undergo dedifferentiation. Expand


Corticosteroid-binding globulin mRNA levels in human uterine endometrium
The findings suggest that CBG is synthesized in the uterine endometrium, predominantly in the secretory phase, and that the serum E2/progesterone ratio exerts an influence on the synthesis of intracellular CBG. Expand
Immunocytochemical localization of corticosteroid-binding globulin in rat tissues.
Investigation of immunocytochemical localization of rat CBG found the presence of immunoreactive CBG in specific cells of some glucocorticoid-responsive tissues and not others raises interesting questions concerning the transport of glucOCorticoids and their mechanism of action. Expand
Role of corticosteroid-binding globulin in interaction of corticosterone with uterine and brain progesterone receptors
It is shown that there are differences between progesterone receptors in brain and uterus, and possibly in the distribution of the serum progestersone-binding protein, corticosteroid-binding globulin (CBG), which may enter uterine but not brain cells. Expand
Stimulation of arylsulfotransferase activity by progestins in human endometrium in vitro.
  • L. Tseng, H. C. Liu
  • Biology, Medicine
  • The Journal of clinical endocrinology and metabolism
  • 1981
Results indicate that arylsulfotransferase in endometrium originates from glandular epithelial cells and can be stimulated by progestin. Expand
Induction of adenylate cyclase in a mammary carcinoma cell line by human corticosteroid-binding globulin.
It is established that binding to specific binding sites for Corticosteroid-Binding globulin results in the induction of adenylate cyclase activity and the accumulation of cAMP in MCF-7 cells, critically dependent upon a steroid being bound to CBG. Expand
Effects of progestins on estradiol receptor levels in human endometrium.
The results indicate that one of the progestin effects on human endometrium is the reduction of E2 receptor levels, which is significantly lower than the average E1 receptor levels in proliferativeendometrium of untreated subjects. Expand
Synthesis and secretion of corticosteroid-binding globulin by rat liver. A source of heterogeneity of hepatic corticosteroid-binders.
In conclusion, liver synthesizes and secretes type III sites, a finding previously suspected but never proved, and the presence oftype III sites in kidney remains to be explained. Expand
An in vivo system in man for quantitation of estrogenicity. I. Physiologic changes in binding capacity of serum corticosteroid-binding globulin.
The findings of this study indicate that serum levels of CBG-BC are relatively constant in men and menstruating women, and in pregnancy after a high threshold of endogenous estrogen is reached, CBg-BC increases in a direct dose-response manner as levels of estradiol increase further. Expand
Endometrial cancer: biochemical and clinical correlates.
  • E. Gurpide
  • Biology, Medicine
  • Journal of the National Cancer Institute
  • 1991
In vivo and in vitro studies on normal and neoplastic tissues are providing evidence of paracrine influences on epithelial cell proliferation, and tamoxifen as adjuvant therapy for breast cancer has recently been found to increase the risk for development of endometrial cancer. Expand
Induction of human endometrial estradiol dehydrogenase by progestins.
In vitro and in vivo results point to progesterone as the agent responsible for the 10-fold increase in endometrial estradiol dehydrogenase activity observed during the luteal phase in menstruating women. Expand