Expression of cloned monkey metallothionein in Escherichia coli.

Abstract

Expression vectors were constructed in which a cDNA specifying the monkey kidney metallothionein-II (MT-II) was linked directly to the lambda PR promoter. Enhanced expression of MT-II in Escherichia coli was observed when two initiation signals were tandemly linked to the MT-II gene and the lambda cI+ host cells were induced by nalidixic acid.

Statistics

050100150'96'98'00'02'04'06'08'10'12'14'16
Citations per Year

287 Citations

Semantic Scholar estimates that this publication has 287 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Murooka1987ExpressionOC, title={Expression of cloned monkey metallothionein in Escherichia coli.}, author={Yoshikatsu Murooka and Tomohiko Nagaoka}, journal={Applied and environmental microbiology}, year={1987}, volume={53 1}, pages={204-7} }