Expression of cloned monkey metallothionein in Escherichia coli.


Expression vectors were constructed in which a cDNA specifying the monkey kidney metallothionein-II (MT-II) was linked directly to the lambda PR promoter. Enhanced expression of MT-II in Escherichia coli was observed when two initiation signals were tandemly linked to the MT-II gene and the lambda cI+ host cells were induced by nalidixic acid.


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@article{Murooka1987ExpressionOC, title={Expression of cloned monkey metallothionein in Escherichia coli.}, author={Yoshikatsu Murooka and Tomohiko Nagaoka}, journal={Applied and environmental microbiology}, year={1987}, volume={53 1}, pages={204-7} }