Expression of chromate resistance genes from Shewanella sp. strain ANA-3 in Escherichia coli.

@article{AguilarBarajas2008ExpressionOC,
  title={Expression of chromate resistance genes from Shewanella sp. strain ANA-3 in Escherichia coli.},
  author={Esther Aguilar-Barajas and Elyse Paluscio and Carlos Cervantes and Christopher Rensing},
  journal={FEMS microbiology letters},
  year={2008},
  volume={285 1},
  pages={97-100}
}
The plasmidic chromate resistance genes chrBAC from Shewanella sp. strain ANA-3 were transferred to Escherichia coli. Expression of chrA alone, on a high- or low-copy number plasmid, conferred increased chromate resistance. In contrast, expression of the complete operon chrBAC on a high-copy number plasmid did not result in a significant increase in resistance, although expression on a low-copy number plasmid made the cells up to 10-fold more resistant to chromate. The chrA gene also conferred… CONTINUE READING

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