Expression of caltrin in the baculovirus system and its purification in high yield and purity by cobalt (II) affinity chromatography.

Abstract

Direct protein extraction from animals is the only approach available to obtain caltrin, calcium transport inhibitor. Here we report the expression and purification of caltrin, previously shown to hinder the influx of calcium into epididymal spermatozoa. Cloning of the caltrin gene into the pCDNA3.1 V5/His-TOPO vector and the subsequent ligation of the… (More)

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