Expression of calf prochymosin gene in Escherichia coli.


The expression plasmid pTaAC containing Tac promoter and calf prochymosin B gene was constructed and transformed into E.coli JM105. Addition of 0.1 mM IPTG into the culture at logarithmic phase induced the production of prochymosin markedly. The expression of prochymosin gene was regulated by temperature in addition to the inducer IPTG. At 30 degrees C in… (More)