Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells.

@article{Sasajima2010ExpressionOA,
  title={Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells.},
  author={Yoshiyuki Sasajima and Ryohei Iwasaki and Kouhei Tsumoto and Izumi Kumagai and Masaki Ihara and Hiroshi Ueda},
  journal={Journal of immunological methods},
  year={2010},
  volume={361 1-2},
  pages={57-63}
}
Antibody fragments and their fusion proteins are indispensable tools as immunoassay reagents in diagnostics and molecular/cellular biotechnology. However, bacterial expression of cloned antibody genes with correct tertiary structure is not always guaranteed because of the lack of proper folding machinery and/or post-translational modifications. In addition, frequently used bacterial alkaline phosphatase as a fusion partner generally shows lower specific activity than the mammalian enzyme, which… CONTINUE READING