Expression of an insecticidal fern protein in cotton protects against whitefly
@article{Shukla2016ExpressionOA, title={Expression of an insecticidal fern protein in cotton protects against whitefly}, author={Anoop Kumar Shukla and Santosh Kumar Upadhyay and Manisha Mishra and Sharad Saurabh and Rahul Mohan Singh and Harpal Singh and Nidhika Thakur and Preeti Rai and Paras Pandey and Aradhana Lucky Hans and Subhi Srivastava and Vikram Rajapure and Sunil Kumar Yadav and Mithlesh Kumar Singh and Jitendra Kumar and K Chandrashekar and Praveen Chandra Verma and Ajit Pratap Singh and K. Narayanan Nair and Smrati Bhadauria and Muhammad Wahajuddin and Sarika Singh and Sharad Sharma and Omkar and Ram Sanmukh Upadhyay and Shirish Anand Ranade and Rakesh Kumar Tuli and Pradhyumna K. Singh}, journal={Nature Biotechnology}, year={2016}, volume={34}, pages={1046-1051} }
Whitefly (Bemisia tabaci) damages field crops by sucking sap and transmitting viral diseases. None of the insecticidal proteins used in genetically modified (GM) crop plants to date are effective against whitefly. We report the identification of a protein (Tma12) from an edible fern, Tectaria macrodonta (Fee) C. Chr., that is insecticidal to whitefly (median lethal concentration = 1.49 μg/ml in in vitro feeding assays) and interferes with its life cycle at sublethal doses. Transgenic cotton…
68 Citations
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References
SHOWING 1-10 OF 24 REFERENCES
Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, Lepidopteran insects, bacterial and viral pathogens.
- BiologyPlant biotechnology journal
- 2012
Broad spectrum resistance to homopteran (sap-sucking), Lepidopteran insects as well as anti-bacterial or anti-viral activity observed in PTA lines provides a new option to engineer protection against biotic stress by hyper-expression of an unique protein that is naturally present in a medicinal plant.
Evaluation of Fern and Moss Protein‐Based Defenses Against Phytophagous Insects
- BiologyInternational Journal of Plant Sciences
- 2006
Protein extracts from ferns, mosses, and the burned ground moss caused the greatest decrease in damage in leaf‐disk assays and insect larval growth, and are good candidates for follow‐up evaluation.
Toxins for Transgenic Resistance to Hemipteran Pests
- BiologyToxins
- 2012
The increased pest status of numerous hemipteran species, combined with increased prevalence of resistance to chemical insecticides, provides impetus for the development of biologically based, alternative management strategies.
Bacillus thuringiensis: a century of research, development and commercial applications.
- BiologyPlant biotechnology journal
- 2011
Recent progress in the development of Bt technology and the countermeasures that have been introduced to prevent the evolution of resistant insect populations are reviewed.
Emerging virus diseases transmitted by whiteflies.
- BiologyAnnual review of phytopathology
- 2011
Factors driving the emergence and establishment of whitefly-transmitted diseases include genetic changes in the virus through mutation and recombination, changes inThe vector populations coupled with polyphagy of the main vector, Bemisia tabaci, and long distance traffic of plant material or vector insects due to trade of vegetables and ornamental plants.
RNA interference for the control of whiteflies (Bemisia tabaci) by oral route
- BiologyJournal of Biosciences
- 2011
Phloem-specific expression of dsRNAs of RPL9 and V-ATPase A in transgenic plants for the protection against whiteflies might be an interesting application of this technology.
Management of Bemisia tabaci Whiteflies
- Biology
- 2011
Adopting IPM will alleviate the numerous concerns that accompany the use of chemicals, including those associated with environmental pollution and the widespread resistance that plagues B. tabaci management.
Enhanced Whitefly Resistance in Transgenic Tobacco Plants Expressing Double Stranded RNA of v-ATPase A Gene
- BiologyPloS one
- 2014
The effectiveness of transgenic tobacco plants expressing siRNA to silence v-ATPaseA gene expression for the control of whitefly infestation is reported, mounting a significant silencing response, leading to their mortality.
Toxin delivery by the coat protein of an aphid-vectored plant virus provides plant resistance to aphids
- BiologyNature Biotechnology
- 2014
It is shown that when four aphid pest species feed on a recombinant coat protein–toxin fusion, either in an experimental membrane sachet or in transgenic Arabidopsis plants, they experience significant mortality.
Field evaluation of three fern extracts on Helicoverpa armigera (Hubner) and Spodoptera litura (Fab.) incidence and infestation and groundnut production
- Biology
- 2013
Field efficacy of three fern extracts were evaluated for their pesticidal property against Helicoverpa arrnigera (Hubner) and Spodoptera litura (Fab.) under groundnut field condition. The fern…