Expression of an exogenous eukaryotic DNA methyltransferase gene induces transformation of NIH 3T3 cells.

@article{Wu1993ExpressionOA,
  title={Expression of an exogenous eukaryotic DNA methyltransferase gene induces transformation of NIH 3T3 cells.},
  author={Julie Michelle Wu and Jean-Pierre Issa and J Herman and Douglas E. Bassett and Barry D. Nelkin and Stephen B. Baylin},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 19},
  pages={8891-5}
}
Abnormal regional increases in DNA methylation, which have potential for causing gene inactivation and chromosomal instability, are consistently found in immortalized and tumorigenic cells. Increased DNA methyltransferase activity, which is also a characteristic of such cells, is a candidate to mediate these abnormal DNA methylation patterns. We now show that, in NIH 3T3 mouse fibroblasts, constitutive overexpression of an exogenous mouse DNA methyltransferase gene results in a marked increase… CONTINUE READING