Expression of an electrically silent voltage-gated potassium channel in the human placenta

  title={Expression of an electrically silent voltage-gated potassium channel in the human placenta},
  author={Gregor K. Fyfe and Sumith R. Panicker and Rebecca L Jones and Mark Wareing},
  journal={Journal of Obstetrics and Gynaecology},
  pages={624 - 629}
Human placental expression of KV9.3, a voltage-gated K channel linked to tissue oxygenation responses, has been suggested at the messenger RNA level but tissue localisation has not been described. We aimed to: (1) produce an antibody to human KV9.3 and (2) assess channel expression and distribution in human placental tissue. We determined human placental protein expression and localisation using an antibody to KV9.3. Antibody specificity was confirmed by Western blotting. Staining was observed… 
Characterisation of K+ Channels in Human Fetoplacental Vascular Smooth Muscle Cells
This study provides the first direct evidence for functional Kv, BKCa, IKCa and SKCa channels in CPASMCs, which display a mixed phenotype implicating a dual role for CPAS MCs in controlling both fetoplacental vascular resistance and vasculogenesis.
Activation of KV7 channels stimulates vasodilatation of human placental chorionic plate arteries.
Kv7 channels are present and active in fetoplacental vessels, contributing to vascular tone regulation in normal pregnancy and targeting these channels may represent a therapeutic intervention in pregnancies complicated by increased vascular resistance.
Oxygen Sensitivity, Potassium Channels, and Regulation of Placental Vascular Tone
This review focuses on the roles of K+ channels and oxygenation in controlling reactivity of small fetoplacental blood vessels.
Review: Potassium channels in the human fetoplacental vasculature.
Current understanding of K channel expression and activity in fetoplacental vasculature in normal and complicated pregnancies is summarized.
Silencing of voltage-gated potassium channel KV9.3 inhibits proliferation in human colon and lung carcinoma cells
Voltage-gated potassium (Kv) channels are known to be involved in cancer development and cancer cell proliferation. KV9.3, an electronically silent subunit, forms heterotetramers with KV2.1 in
Kv 5 , Kv 6 , Kv 8 , and Kv 9 subunits : No simple silent bystanders
Kv2 channels The voltage-gated K (Kv) channel Kv2.1 plays a crucial role in many cell types, with its contribution to various processes depending on both its conductive and nonconductive properties.
NADPH oxidase is the major source of placental superoxide in early pregnancy: association with MAPK pathway activation
Nox is the major superoxide source in early pregnancy and increased superoxide production at 7–9 GW is associated with p38 MAPK pathway activation, suggesting that it is involved in physiological placental function and healthy early development of the placenta, through MAPK pathways.
Kv5, Kv6, Kv8, and Kv9 subunits: No simple silent bystanders
  • E. Bocksteins
  • Biology, Medicine
    The Journal of general physiology
  • 2016
An overview of the expression of KvS subunits in different tissues is provided and their proposed role in various physiological and pathophysiological processes are discussed and the importance of considering Kv2/KvS heterotetramers in the development of novel treatments is demonstrated.
Clotting factor genes are associated with preeclampsia in high altitude pregnant women in the Peruvian Andes
The discovery of a novel association related to a functional pathway relevant to pregnancy biology in an understudied population of Native American origin demonstrates the increased power of family-based study design and underscores the importance of conducting genetic research in diverse populations.


Expression and function of potassium channels in the human placental vasculature.
  • M. Wareing, X. Bai, +5 authors G. K. Fyfe
  • Biology, Medicine
    American journal of physiology. Regulatory, integrative and comparative physiology
  • 2006
It is concluded that K channels play an important role in controlling placental vascular function, and pharmacological manipulation of voltage-gated and ATP-sensitive channels produced the most marked modifications in vascular tone, in both arteries and veins.
Altered Potassium Channel Expression in the Human Placental Vasculature of Pregnancies Complicated by Fetal Growth Restriction
The gene expression of two voltage-gated K channels thought to be important for altered vascular reactivity following changes in oxygenation is increased in FGR, reducing hypoxia-induced contraction of pulmonary vascular tissue.
Functional evidence for oxygen-sensitive voltage-gated potassium channels in human placental vasculature.
Using wire myography, chorionic plate blood vessels were exposed to isoform-specific K(V) channel blockers and HFPV-induced contraction increased with margatoxin and stromatoxin-1, whilst only correolide increased U46619- induced contraction in veins.
Oxygen sensitivity of cloned voltage-gated K(+) channels expressed in the pulmonary vasculature.
These results indicate that Kv1.2 and Kv2.3 heteromeric channels are strong candidates for the K(+) channel isoforms initiating hypoxic pulmonary vasoconstriction.
Expression of the Kir2.1 (inwardly rectifying potassium channel) gene in the human placenta and in cultured cytotrophoblast cells at different stages of differentiation.
Data demonstrate that the Kir2.1 gene is expressed by the human placenta and, specifically, by cytotrophoblast cells, at all stages of development and differentiation.
Barium, TEA and sodium sensitive potassium channels are present in the human placental syncytiotrophoblast apical membrane.
The results show the existence of at least two types of Ba(+2)-sensitive K(+) channels including a TEA sensitive sub-population, and some of them Na(+) sensitive K(-) channels.
Kv2.1/Kv9.3, an ATP‐Dependent Delayed‐Rectifier K+ Channel in Pulmonary Artery Myocytes
It is observed that outward K currents in PA smooth muscle cells are relatively resistant to tetraethylammonium (TEA), but sensitive to 4-aminopyridine (4-AP), which has been shown to induce PA vasoconstriction.
Voltage‐gated K channels support proliferation of colonic carcinoma cells
The data suggest that Kv channels control proliferation of colonic cancer cells by affecting intracellular pH and Ca2+ signaling.
Hypoxic fetoplacental vasoconstriction in humans is mediated by potassium channel inhibition.
It is concluded that human fetoplacental vessels constrict in response to hypoxia, largely mediated by hypoxic inhibition of K(v) channels in the smooth muscle of small fetoplACental arteries.
Molecular basis and function of voltage-gated K+ channels in pulmonary arterial smooth muscle cells.
The data suggest that native K+ channels in PASMC are encoded by multiple genes, and the A-type I K(V) may be generated by the KV1.4 channel and/or the delayed rectifier KV channels (KV1 subfamily) associated with β-subunits.