Expression of an antibody fragment at high levels in the bacterial cytoplasm.

  title={Expression of an antibody fragment at high levels in the bacterial cytoplasm.},
  author={Patrick Martineau and Peter Jones and Greg Winter},
  journal={Journal of molecular biology},
  volume={280 1},
Recombinant antibody fragments expressed in the cytoplasm of cells have considerable practical potential. However in the reducing environment of the cytoplasm, the intradomain disulphide bonds are not formed and the fragments are unstable and expressed in low yields. Here we attempted to overcome these limitations. We first isolated an antibody single chain Fv fragment that binds and activates an inactive mutant beta-galactosidase. We then subjected the gene encoding the scFv fragment to random… CONTINUE READING
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