Expression of an anti-CD3 single-chain immunotoxin with a truncated diphtheria toxin in a mutant CHO cell line.

@article{Liu2000ExpressionOA,
  title={Expression of an anti-CD3 single-chain immunotoxin with a truncated diphtheria toxin in a mutant CHO cell line.},
  author={Yuan Yi Liu and I. Ju. Gordienko and Askale Mathias and S-F. Ma and Jerry T Thompson and Jung Hee Woo and David M. Neville},
  journal={Protein expression and purification},
  year={2000},
  volume={19 2},
  pages={304-11}
}
ADP-ribosylating immunotoxins are generally expressed in Escherichia coli and then refolded in vitro. Because the efficiency of the in vitro refolding process decreases with the number of protein domains and internal disulfide bonds, these immunotoxins have been generally limited to single-chain monovalent structures. We now show that using the hamster cell… CONTINUE READING