Expression of an active recombinant lysine 49 phospholipase A(2) myotoxin as a fusion protein in bacteria.

@article{Giuliani2001ExpressionOA,
  title={Expression of an active recombinant lysine 49 phospholipase A(2) myotoxin as a fusion protein in bacteria.},
  author={Carol Giuliani and Maurizio Iemma and Ana Cristina Vigliar Bondioli and Dulce Helena Ferreira Souza and Leonardo L. Ferreira and Alessandra Cintra Amaral and Tania de F{\'a}tima Salvini and Heloisa S Selistre-de-Ara{\'u}jo},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={2001},
  volume={39 10},
  pages={1595-600}
}
ACL myotoxin (ACLMT) is a K49 phospholipase A(2)-like protein isolated from the venom of the snake Agkistrodon contortrix laticinctus (broad-banded copperhead) that induces necrosis of skeletal muscle. We have previously cloned and sequenced the cDNA coding for ACLMT from a venom gland cDNA library. In order to perform structure and function studies, we have developed an expression system for production of ACLMT as a fusion protein with maltose binding protein (MBP) from the periplasm of… CONTINUE READING

Connections & Topics

Mentioned Connections BETA
The fusion protein was purified from the bacterial periplasm by affinity chromatography in an amylose column and by gel filtration .
The fusion protein was purified from the bacterial periplasm by affinity chromatography in an amylose column and by gel filtration .
All Topics