Expression of an Arabidopsis sodium/proton antiporter gene (AtNHX1) in peanut to improve salt tolerance

  title={Expression of an Arabidopsis sodium/proton antiporter gene (AtNHX1) in peanut to improve salt tolerance},
  author={Manoj Banjara and Longfu Zhu and Guoxin Shen and Paxton R. Payton and Hong Zhang},
  journal={Plant Biotechnology Reports},
Salinity is a major environmental stress that affects agricultural productivity worldwide. One approach to improving salt tolerance in crops is through high expression of the Arabidopsis gene AtNHX1, which encodes a vacuolar sodium/proton antiporter that sequesters excess sodium ion into the large intracellular vacuole. Sequestering cytosolic sodium into the vacuoles of plant cells leads to a low level of sodium in cytosol, which minimizes the sodium toxicity and injury to important enzymes in… 

AtNHX 1 and AtNHX 3 from Arabidopsis improve salt and drought tolerance in transgenic poplar

The tonoplast and plasma membrane localized sodium (potassium)/proton antiporters have been shown to play an important role in plant resistance to salt stress. In this study, AtNHX1 and AtNHX3, two

Overexpression of Sorghum plasma membrane-bound Na+/H+ antiporter-like protein (SbNHXLP) enhances salt tolerance in transgenic groundnut (Arachis hypogaea L.)

Findings indicate that overexpression of SbNHXLP gene in groundnut results in enhanced tolerance to salinity stress, which highlights the potential of S bNHxLP as a target candidate gene to impart salt stress tolerance in groundnuts.

Na+/H+ and K+/H+ antiporters AtNHX1 and AtNHX3 from Arabidopsis improve salt and drought tolerance in transgenic poplar

Constutive expression of either At NHX1 or AtNHX3 genetically modified the salt and water stress tolerance of transgenic poplar plants, providing a potential tool for engineering tree species with enhanced resistance to multiple abitotic stresses.

Elevated compartmentalization of Na+ into vacuoles improves salt and cold stress tolerance in sweet potato (Ipomoea batatas).

It is demonstrated that overexpressing AtNHX1 in sweet potato renders the crop tolerant to both salt and cold stresses, providing a greater capacity for the use of AtNHx1 in improving crop performance under combined abiotic stress conditions.

Co-overexpressing a Plasma Membrane and a Vacuolar Membrane Sodium/Proton Antiporter Significantly Improves Salt Tolerance in Transgenic Arabidopsis Plants

Co-overexpression of AtNHX1 and SOS1 could significantly reduce yield loss caused by the combined stresses of heat and salt, confirming the hypothesis that stacked overexpression of two genes could substantially improve tolerance against multiple stresses.

Enhanced salinity tolerance in transgenic mungbean overexpressing Arabidopsis antiporter (NHX1) gene

Results indicated that the activity of heterologous AtNHX1 protein contributing enhanced salt tolerance in transgenic mungbean lines showed reduced membrane lipid peroxidation and H2O2 and O2− accumulation, higher levels of antioxidant enzyme activity and increased accumulation of proline and ascorbate than WT.

A Glycine max sodium/hydrogen exchanger enhances salt tolerance through maintaining higher Na+ efflux rate and K+/Na+ ratio in Arabidopsis

Vacuolar membrane-localized GmNHX1 enhances plant salt tolerance through maintaining a high K+/Na+ ratio along with inducing the expression of SKOR, SOS1 and AKT1 and the transcription levels of three osmotic stress-related genes were all up-regulated in Gm NHX1-expressing plants under salt stress condition.

Ectopic overexpression of a mungbean vacuolar Na+/H+ antiporter gene (VrNHX1) leads to increased salinity stress tolerance in transgenic Vigna unguiculata L. Walp

Under salt stress conditions, T2 transgenic 35S:VrNHX1 cowpea lines exhibited higher tolerance to 200 mM NaCl treatment than wild-type and maintained a higher K+/Na+ ratio in the aerial parts under salt stress and accumulated higher [Na+] in roots than wild -type.

Improved salinity tolerance and growth performance in transgenic sunflower plants via ectopic expression of a wheat antiporter gene (TaNHX2)

It is shown here that the over-expression of wheat TaNHX2 gene in transgenic sunflower conferred improved salinity stress tolerance and growth performance and this expression contributed towards improving growth performance under sodium chloride stress.



Expression of an Arabidopsis vacuolar sodium/proton antiporter gene in cotton improves photosynthetic performance under salt conditions and increases fiber yield in the field.

Overexpression of AtNHX1 increases sodium uptake in vacuoles, which leads to increased vacuolar solute concentration and therefore higher salt tolerance in transgenic plants, and indicates that At NHX1 can indeed be used for improving salt stress tolerance in cotton.

Expression of an Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1) in cotton improves drought- and salt tolerance and increases fibre yield in the field conditions.

Overexpression of AVP1 in Arabidopsis, tomato and rice enhances plant performance under salt and drought stress conditions, and has the potential to be used for improving crop's drought- and salt tolerance in areas where water and salinity are limiting factors for agricultural productivity.

Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis.

Overexpression of a vacuolar Na+/H+ antiport fromArabidopsis thaliana in Arabidopsis plants promotes sustained growth and development in soil watered with up to 200 millimolar sodium chloride, demonstrating the feasibility of engineering salt tolerance in plants.

Overexpression of AtNHX1, a Vacuolar Na+/H+ Antiporter from Arabidopsis thalina, in Petunia hybrida Enhances Salt and Drought Tolerance

It was demonstrated that overexpression of the vacuolar Na+/H+ antiporter conferred both salt tolerance and drought tolerance to the transgenic Petunia plants.

Stable expression of Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1, and salt tolerance in transgenic soybean for over six generations

The results indicated that constitutive expression of AtNHX1 enhanced salt tolerance in soybean for over 6 generations, suggesting a great potential use of At NHX1 for improving saltolerance in plants by genetic engineering.

Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump

  • R. GaxiolaJisheng Li G. Fink
  • Environmental Science
    Proceedings of the National Academy of Sciences of the United States of America
  • 2001
The phenotypes of the AVP1 transgenic plants suggest that increasing the vacuolar proton gradient results in increased solute accumulation and water retention, and sequestration of cations in the vacUole reduces their toxic effects.

Overexpression AtNHX1 confers salt-tolerance of transgenic tall fescue

It is reported that over-expression of AtNHX1 improves salt tolerance in transgenic tall fescue and the exogenous genes had been integrated into the genomes of transgenic plants, and At NHX1 is expressed in the plants.

Engineering salt-tolerant Brassica plants: Characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation

The findings, showing that the modification of a single trait significantly improved the salinity tolerance of this crop plant, suggest that with a combination of breeding and transgenic plants it could be possible to produce salt-tolerant crops with far fewer target traits than had been anticipated.

The transgene pyramiding tobacco with betaine synthesis and heterologous expression of AtNHX1 is more tolerant to salt stress than either of the tobacco lines with betaine synthesis or AtNHX1.

Based on the integrative analysis of salt tolerance, the consistency between the cellular level and the whole plant level was confirmed and the transgene pyramiding plants exhibited improved saltolerance, but compared with the plants with betA or AtNHX1 alone, the differences were relatively small.

The Putative Plasma Membrane Na+/H+ Antiporter SOS1 Controls Long-Distance Na+ Transport in Plants Article, publication date, and citation information can be found at

The salt tolerance locus SOS1 from Arabidopsis has been shown to encode a putative plasma membrane Na+/H+ antiporter, and a model in which SOS1 functions in retrieving Na+ from the xylem stream under severe salt stress, whereas under mild salt stress it may function in loading Na+ into the Xylem.