Expression of allograft inflammatory factor-1 in inflammatory skin disorders.


Allograft inflammatory factor-1 (AIF-1) is an evolutionarily conserved, inflammatory protein produced by activated macrophages during chronic transplant rejection and in inflammatory brain lesions. Since T-cell-mediated inflammation is common to various dermatoses and nothing is known about AIF-1 in skin, we studied its protein expression at the tissue level and regulation in monocytic cell lines by various agents. Using immunohistochemistry, we found that AIF-1 is expressed at low levels in normal skin, but is highly upregulated in various inflammatory skin disorders, such as psoriasis, lichen planus, graft-versus-host disease and mycosis fungoides. The main cell types expressing AIF-1 in affected skin are macrophages and Langerhans' cells. We also show by real-time PCR that AIF-1 mRNA levels in monocytic THP-1 and U937 cell lines are significantly upregulated by retinoic acid as well as a number of cytokines. We conclude that AIF-1 may mediate survival and pro-inflammatory properties of macrophages in skin diseases.

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@article{Orsmark2007ExpressionOA, title={Expression of allograft inflammatory factor-1 in inflammatory skin disorders.}, author={Christina Orsmark and Tiina Skoog and Leila Jeskanen and Juha Kere and Ulpu K Saarialho-Kere}, journal={Acta dermato-venereologica}, year={2007}, volume={87 3}, pages={223-7} }