Expression of active Streptomyces phage phiC31 integrase in transgenic wheat plants

@article{Rubtsova2008ExpressionOA,
  title={Expression of active Streptomyces phage phiC31 integrase in transgenic wheat plants},
  author={Myroslava Rubtsova and Katja Kempe and Angelika Gils and Ainur Ismagul and Jens Weyen and Mario Gils},
  journal={Plant Cell Reports},
  year={2008},
  volume={27},
  pages={1821-1831}
}
Site-specific recombination systems are becoming an important tool for the genetic modification of crop plants. Here we report the functional expression of the Streptomyces phage-derived phiC31 recombinase (integrase) in wheat. T-DNA constructs containing a phiC31 integrase transgene were stably transformed into wheat plants via particle gun bombardment. A plant-virus-based assay system was used to monitor the site-specific recombination activity of the recombinant integrase protein in vivo. We… CONTINUE READING

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