Expression of a thermostable xylanase gene from Bacillus coagulans ST-6 in Lactococcus lactis.

@article{Raha2006ExpressionOA,
  title={Expression of a thermostable xylanase gene from Bacillus coagulans ST-6 in Lactococcus lactis.},
  author={Abdul Rahim Raha and Li Yun Chang and A Sipat and Khatijah Yusoff and Titin Haryanti},
  journal={Letters in applied microbiology},
  year={2006},
  volume={42 3},
  pages={210-4}
}
AIMS The aim of the study is to evaluate whether xylanase can be used as a potential reporter gene for cloning and expression studies in Lactococcus. METHODS AND RESULTS The 750 bp xylanase gene was amplified and subcloned into the unique NheI restriction enzyme site of pMG36e and subsequently transformed into competent Escherichia coli XLI-blue MRF cells and Lactococcus lactis cells. Bacterial culture containing pMG36e-Xy has an enzyme activity of 390 microg xylose ml(-1) culture 30 min(-1… CONTINUE READING

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