Expression of a synthetic copy of the bovine chymosin gene in Aspergillus awamori from constitutive and pH-regulated promoters and secretion using two different pre-pro sequences.

@article{Cardoza2003ExpressionOA,
  title={Expression of a synthetic copy of the bovine chymosin gene in Aspergillus awamori from constitutive and pH-regulated promoters and secretion using two different pre-pro sequences.},
  author={Rosa E Cardoza and Santiago Guti{\'e}rrez and Nely Ortega and Ana R Colina and Javier Casqueiro and Juan F. Martin},
  journal={Biotechnology and bioengineering},
  year={2003},
  volume={83 3},
  pages={249-59}
}
A copy of the bovine chymosin gene (chy) with a codon usage optimized for its expression in Aspergillus awamori was constructed starting from synthetic oligonucleotides. To study the ability of this filamentous fungus to secrete bovine prochymosin, two plasmids were constructed in which the transcriptional, translational, and secretory control regions of the A. nidulans gpdA gene and pepB genes were coupled to either preprochymosin or prochymosin genes. Secretion of a protein enzymatically and… CONTINUE READING

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