Expression of a single transfected cDNA converts fibroblasts to myoblasts

@article{Davis1987ExpressionOA,
  title={Expression of a single transfected cDNA converts fibroblasts to myoblasts},
  author={Robert Lesley Davis and Harold Weintraub and Andrew B. Lassar},
  journal={Cell},
  year={1987},
  volume={51},
  pages={987-1000}
}

MyoD1: a nuclear phosphoprotein requiring a Myc homology region to convert fibroblasts to myoblasts.

Exposure of a complementary DNA (cDNA) encoding the mouse MyoD1 protein in a variety of fibroblast and adipoblast cell lines converts them to myogenic cells and expression of only 68 amino acids of Myo D1 is sufficient to activate myogenesis in stably transfected 10T1/2 cells.

Differential expression of two distinct MyoD genes in Xenopus.

  • J. B. ScalesE. OlsonM. Perry
  • Biology
    Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
  • 1991
The absence of apparent effect of the maternally expressed myogenic gene in early embryos, but not in transfected fibroblasts, suggests the existence of regulatory mechanisms that repress the function of this gene in cells with nonmuscle fates during early amphibian development.

Differential Expression of Two Distinct MyoD Genes in Xenopus 1

The absence of apparent effect of the maternally expressed myogenic gene in early embryos, but not in transfected fibroblasts, suggests the existence of regulatory mechanisms that repress the function of this gene in cells with nonmuscle fates during early amphibian development.

Disruption of the coordinate expression of muscle genes in a transfected BC3H1 myoblast cell line producing a low level of the adenovirus E1A transforming protein.

Mouse BC3H1 myoblasts stably transfected with the adenovirus 5 E1A gene and one clonal line, BC3E7, was found to differ in some important respects, suggesting that different levels of E 1A may be needed to repress different promoters and that E1a does not block myogenic differentiation by repressing myogenin expression, but represses each muscle gene independently.

A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program.

The existence of a family of myogenic regulatory genes that share a conserved motif with comyc is suggested, suggesting the existence of the muscle differentiation program and may substitute for MyoD1 in certain developmental situations.

Cloning and characterization of a myoblast cell surface antigen defined by 24.1 D5 monoclonal antibody

24.1D5 mRNAs were expressed by multipotential cells and myoblast derivatives of the mouse embryonic cell line C3H10Ti, suggesting that 24.1 D5 is expressed at an early stage during skeletal muscle development.

Methylation and expression of the Myo D1 determination gene.

Preliminary observations suggest that Myo D1 is not methylated at CCGG sites in vivo so that a de novo methylation event may have occurred in vitro, which may have significance in the establishment of immortal cell lines and tumours.
...

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Transfection of a DNA locus that mediates the conversion of 10T1 2 fibroblasts to myoblasts

Constitutive c-myc oncogene expression blocks mouse erythroleukaemia cell differentiation but not commitment

To study the influence of oncogene activation on differentiation, transfected viral-promoter-driven c-myc genes into mouse erythroleukaemia cells andConstitutive c- myc expression was found to block DMSO-induced differentiation.

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The selection of cells able to differentiate in the presence of BUdR may provide a general approach for increasing the expression of the regulatory molecules controlling terminal differentiation.

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It is concluded that H36 is a muscle- specific, developmentally regulated cell-surface antigen that may have a role in myoblast differentiation and that can be used to determine the embryonic lineages of skeletal and cardiac muscle.

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