Expression of a renin/GFP transgene in mouse embryonic, extra-embryonic, and adult tissues.

@article{Jones2000ExpressionOA,
  title={Expression of a renin/GFP transgene in mouse embryonic, extra-embryonic, and adult tissues.},
  author={C. A. Jones and M. I. Hurley and Thomas A. Black and Colleen M. Kane and Li Pan and Steven C Pruitt and Kenneth W. Gross},
  journal={Physiological genomics},
  year={2000},
  volume={4 1},
  pages={
          75-81
        }
}
A reporter construct was assembled with 4-kb of renin 5'-flanking sequence fused to humanized green fluorescent protein (GFP) cDNA. Transgenic mice carrying this construct were produced and assayed for GFP expression. In the adult, expression was detected in juxtaglomerular (JG) cells of the kidney and granular convoluted tubular cells of the submandibular gland. Furthermore, treatment of mice with captopril induced GFP expression in renal vascular smooth muscle cells. During embryogenesis, GFP… 
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Distribution of cells expressing human renin-promoter activity in the brain of a transgenic mouse
Reporter gene recombination in juxtaglomerular granular and collecting duct cells by human renin promoter-Cre recombinase transgene.
TLDR
The data indicate that besides its well-known activity in JG cells and renal vessels the human renin promoter is transiently active in the collecting duct system during kidney development, complicating the use of this approach for JG cell-specific excision of floxed targets.
Expression of renin in large arteries outside the kidney revealed by human renin promoter/LacZ transgenic mouse.
Localization of renin expressing cells in the brain, by use of a REN-eGFP transgenic model.
TLDR
EGFP-containing cells in specific areas of the brain are identified and these findings strongly support the primary expression of renin mRNA in the brain in regions controlling cardiovascular function.
Using a Dual-Reporter Transgenic Model Adjacent Expression of Renin and Angiotensinogen in the Rostral Ventrolateral Medulla
TLDR
Both renin and angiotensinogen are coexpressed in the parabrachial nucleus and central nucleus of the amygdala and are in adjacent cells in the RVLM, reticular formation, bed nucleus ofthe stria terminalis, subfornical organ, and CA1–3 region.
Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium.
TLDR
It is shown that Notch signaling and the endothelium are essential for developmental renin expression, and after inhibition of angiogenesis, renin-expressing cells precede angiogenic sprouts.
A minimal Ksp-cadherin promoter linked to a green fluorescent protein reporter gene exhibits tissue-specific expression in the developing kidney and genitourinary tract.
TLDR
Results demonstrate that 324 bp of the Ksp-cadherin 5' flanking region is sufficient to direct epithelial-specific expression in the developing kidney and GU tract.
Adjacent Expression of Renin and Angiotensinogen in the Rostral Ventrolateral Medulla Using a Dual-Reporter Transgenic Model
TLDR
Both renin and angiotensinogen are coexpressed in the parabrachial nucleus and central nucleus of the amygdala and are in adjacent cells in the RVLM, reticular formation, bed nucleus ofthe stria terminalis, subfornical organ, and CA1–3 region.
Zebrafish mesonephric renin cells are functionally conserved and comprise two distinct morphological populations.
TLDR
Transcriptional responses of ren to physiological challenge support the presence of a functional renin-angiotensin system and are consistent with the production of active renin, demonstrating substantive conservation of renin regulation across vertebrates.
FLUORESCENCE ACTIVATED CELL SORTING OF TRANSIENTLY TRANSFECTED As4.1 CELLS SHOWS RENIN ENHANCER DIRECTS ON/OFF SWITCHING OF RENIN PROMOTER IN VITRO
  • B. Morris
  • Biology
    Clinical and experimental pharmacology & physiology
  • 2008
TLDR
Results from the in vitro system used suggest that the Ren‐1c enhancer does not regulate the rate of promoter activity, but rather increases the probability of achieving an active transcriptional state.
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References

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TLDR
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TLDR
The observed ability of renal vascular cells to be recruited to express both renin and T-antigen suggests a mechanism that can explain the development of the renal pathology in these mice.
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TLDR
It is concluded that angiotensin-converting enzyme is necessary to preserve normal kidney architecture and the normal pattern of renin expression and renin mRNA was increased more than 32-fold in the mutant mice compared with wild types.
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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