Expression of a recombinant chimeric protein of hepatitis A virus VP1-Fc using a replicating vector based on Beet curly top virus in tobacco leaves and its immunogenicity in mice

@article{Chung2011ExpressionOA,
  title={Expression of a recombinant chimeric protein of hepatitis A virus VP1-Fc using a replicating vector based on Beet curly top virus in tobacco leaves and its immunogenicity in mice},
  author={Ho Yong Chung and Hyun Ho Lee and Kyung Il Kim and Ha Young Chung and Jeon Hwang-Bo and Jong Hwa Park and Garry Sunter and Jong Bum Kim and Dong Hwa Shon and Won Yong Kim and In Sik Chung},
  journal={Plant Cell Reports},
  year={2011},
  volume={30},
  pages={1513-1521}
}
We describe the expression and immunogenicity of a recombinant chimeric protein (HAV VP1-Fc) consisting of human hepatitis A virus VP1 and an Fc antibody fragment using a replicating vector based on Beet curly top virus (BCTV) in Agrobacterium-infiltrated Nicotiana benthamiana leaves. Recombinant HAV VP1-Fc was expressed with a molecular mass of approximately 68 kDa. Recombinant HAV VP1-Fc, purified using Protein A Sepharose affinity chromatography, elicited production of specific IgG… CONTINUE READING

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