Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae.

  title={Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae.},
  author={Brigitte Twelkmeyer and Peter K Burstr{\"o}m and Jianjun Li and E Richard Moxon and Derek W Hood and Elke K. H. Schweda},
  journal={Carbohydrate research},
  volume={346 13},
The structure of lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strains 1008 and 1247 has been investigated by mass spectrometry and NMR analyses on O-deacylated LPS and core oligosaccharide material. Both strains express the conserved triheptosyl inner core, [l-α-d-Hepp-(1→2)-[PEtn→6]-l-α-d-Hepp-(1→3)-l-α-d-Hepp-(1→5)-[PPEtn→4]-α-Kdo-(2→6)-Lipid A] with PCho→6)-β-d-Glcp (GlcI) substituting the proximal heptose (HepI) at O-4. Strain 1247 expresses the common… 
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Diversity-oriented synthesis of inner core oligosaccharides of the lipopolysaccharide of pathogenic Gram-negative bacteria.
This work synthesized a range of LPS inner core oligosaccharides from a variety of pathogenic bacteria including Y. pestis, H. influenzae, and Proteus that cause plague, meningitis, and severe wound infections and explored their antigenic and immunogenic properties as well as potential applications such as diagnostic tools and vaccines.


A new structural type for Haemophilus influenzae lipopolysaccharide. Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 486.
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Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae.
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Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003.
Structural analysis of the lipopolysaccharide (LPS) of nontypeable Haemophilus influenzae strain 1003 has been achieved by the application of high-field NMR techniques, ESI-MS, capillary
An alternate pattern for globoside oligosaccharide expression in Haemophilus influenzae lipopolysaccharide: structural diversity in nontypeable strain 1124.
Structural studies of LPS from nontypeable H. influenzae strain 1124 expressing motifs linked to both the proximal heptose (HepI) and HepIII at the same time and results for the lpsA mutant indicate that sialyllactose extends from HepI as well, a molecular environment for sialylactose in H. Influenzae that has not been reported previously.
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Lipid A isolated by mild acid hydrolysis from lipopolysaccharides of 22 nontypeable and 2 type f Haemophilus influenzae strains was investigated using electrospray ionization coupled to quadrupole ion trap mass spectrometry to find structures comprising the hexaacylated lipid A with the C2 or C3 position being substituted by 3-hydroxydecanoic acid.
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Electrospray-ionization mass spectrometry on O-deacylated LPS obtained after treatment with anhydrous hydrazine gave evidence for the presence of two minor compounds, which show additional substitution of the main structure with phosphate and PEtn, respectively.
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