Expression of a human multidrug resistance cDNA (MDR1) in the bone marrow of transgenic mice: resistance to daunomycin-induced leukopenia.

@article{Galski1989ExpressionOA,
  title={Expression of a human multidrug resistance cDNA (MDR1) in the bone marrow of transgenic mice: resistance to daunomycin-induced leukopenia.},
  author={Hanan Galski and Margery Sullivan and Mark C. Willingham and K V Chin and Michael M. Gottesman and Ira Pastan and Glenn T. Merlino},
  journal={Molecular and cellular biology},
  year={1989},
  volume={9 10},
  pages={4357-63}
}
The human multidrug resistance gene (MDR1) encodes a drug efflux pump glycoprotein (P-glycoprotein) responsible for resistance to multiple cytotoxic drugs. A plasmid carrying a human MDR1 cDNA under the control of a chicken beta-actin promoter was used to generate transgenic mice in which the transgene was mainly expressed in bone marrow and spleen. Immunofluorescence localization studies showed that P-glycoprotein was present on bone marrow cells. Furthermore, leukocyte counts of the… CONTINUE READING