Expression of a foreign gene by recombinant canine distemper virus recovered from cloned DNAs.

@article{Parks2002ExpressionOA,
  title={Expression of a foreign gene by recombinant canine distemper virus recovered from cloned DNAs.},
  author={Christopher L. Parks and Hai-Ping Wang and Gerald R. Kovacs and Nikos Vasilakis and Jacek Kowalski and Rebecca M Nowak and Robert A. Lerch and Pramila Walpita and Mohinderjit S. Sidhu and Stephen A. Udem},
  journal={Virus research},
  year={2002},
  volume={83 1-2},
  pages={
          131-47
        }
}
A canine distemper virus (CDV) genomic cDNA clone and expression plasmids required to establish a CDV rescue system were generated from a laboratory-adapted strain of the Onderstepoort vaccine virus. In addition, a CDV minireplicon was prepared and used in transient expression studies performed to identify optimal virus rescue conditions. Results from the transient expression experiments indicated that minireplicon-encoded reporter gene activity was increased when transfected cell cultures were… CONTINUE READING
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