Expression of a dominant negative allele of cdc2 prevents activation of the endogenous p34cdc2 kinase

@article{Fleig1991ExpressionOA,
  title={Expression of a dominant negative allele of cdc2 prevents activation of the endogenous p34cdc2 kinase},
  author={Ursula N. Fleig and P. Nurse},
  journal={Molecular and General Genetics MGG},
  year={1991},
  volume={226},
  pages={432-440}
}
The cdc2 gene of the fission yeast Schizosaccharomyces pombe encodes a 34 kDa phosphoprotein with serine/threonine protein kinase activity that acts as the key component in regulation of the eukaryotic cell cycle. We used a repressible promoter fused to the cdc2 cDNA to isolate conditionally dominant negative mutants of cdc2. One of these mutants, DL5, is described in this paper. Overexpression of the mutant protein in a wild-type cdc2 background is lethal and confers cell cycle arrest with a… CONTINUE READING
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Dephosphory - lation and activation of Xenopus p 34 ° a ° 2 protein kinase during the cell cycle

  • KL Gould, P Nurse
  • Nature
  • 1989

Dephosphorylation and activation of Xenopus p34 °a°2 protein kinase during the cell

  • J Gautier, T Matusukawa, P Nurse, J Maller
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