Expression of a chimeric uidA gene indicates that polycistronic mRNAs are efficiently translated in tobacco plastids.

Abstract

Polycistronic mRNAs are the predominant form of plastid primary transcripts. To determine if there is internal initiation of translation of promoter-distal open reading frames, a promoterless uidA reporter gene was integrated into the tobacco plastid genome downstream of the rbcL gene. Monocistronic uidA mRNA does not accumulate from the promoterless uidA… (More)

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