Expression of a calmodulin methylation mutant affects the growth and development of transgenic tobacco plants.

@article{Roberts1992ExpressionOA,
  title={Expression of a calmodulin methylation mutant affects the growth and development of transgenic tobacco plants.},
  author={Daniel M Roberts and Linda Besl and Sang Hwan Oh and Robert V. Masterson and J. L. Schell and Gary Stacey},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 17},
  pages={8394-8}
}
Transgenic plants were constructed that express two foreign calmodulins (VU-1 and VU-3 calmodulins) derived from a cloned synthetic calmodulin gene. VU-1 calmodulin, similar to endogenous plant calmodulin, possesses a lysine residue at position 115 and undergoes posttranslational methylation. VU-3 calmodulin is a site-directed mutant of VU-1 calmodulin that is identical in sequence except for the substitution of an arginine at position 115 and thus is incapable of methylation. Both calmodulin… CONTINUE READING