Expression of a bacterial alpha-amylase gene in transgenic rice seeds.

@article{Xu2008ExpressionOA,
  title={Expression of a bacterial alpha-amylase gene in transgenic rice seeds.},
  author={Xiaoli Xu and Jun Fang and Wei Wang and Jianli Guo and Pinnan Chen and Jiaan Cheng and Zhicheng Shen},
  journal={Transgenic research},
  year={2008},
  volume={17 4},
  pages={645-50}
}
An alpha-amylase gene from Bacillus stearothermophilus under the control of the promoter of a major rice-seed storage protein was introduced into rice. The transgenic line with the highest alpha-amylase activity reached about 15,000 U/g of seeds (one unit is defined as the amount of enzyme that produces 1 mumol of reducing sugar in 1 min at 70 degrees C). The enzyme produced in the seeds had an optimum pH of 5.0-5.5 and optimum temperature of 60-70 degrees C. Without extraction or purification… CONTINUE READING