Expression of a Thermomonospora fusca Cellulase Gene in Streptomyces lividans and Bacillus subtilis.

@article{Ghangas1987ExpressionOA,
  title={Expression of a Thermomonospora fusca Cellulase Gene in Streptomyces lividans and Bacillus subtilis.},
  author={G. S. Ghangas and David B. Wilson},
  journal={Applied and environmental microbiology},
  year={1987},
  volume={53 7},
  pages={1470-5}
}
A cellulase gene from Thermomonospora fusca coding for endocellulase E(5) was introduced into Streptomyces lividans by using shuttle plasmids that can replicate in either S. lividans or Escherichia coli. Plasmid DNA isolated from E. coli was used to transform S. lividans, selecting for thiostrepton resistance. The transformants expressed and excreted the endocellulase, but the ability to produce the endocellulase was unstable. This instability was shown to result from deletion of the… CONTINUE READING