Expression of a Phanerochaete chrysosporium manganese peroxidase gene in the yeast Pichia pastoris.

@article{Gu2003ExpressionOA,
  title={Expression of a Phanerochaete chrysosporium manganese peroxidase gene in the yeast Pichia pastoris.},
  author={Lina Gu and Curtis A Lajoie and Christine Kelly},
  journal={Biotechnology progress},
  year={2003},
  volume={19 5},
  pages={1403-9}
}
A gene encoding manganese peroxidase (mnp1) from Phanerochaete chrysosporium was cloned downstream of a constitutive glyceraldehyde-3-phosphate dehydrogenase promoter in the methylotrophic yeast Pichia pastoris. Three different expression vectors were constructed: pZBMNP contains the native P. chrysosporium fungal secretion signal, palphaAMNP contains an alpha-factor secretion signal derived from Saccharomyces cerevisiae, and pZBIMNP has no secretion signal and was used for intracellular… CONTINUE READING

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