Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector

@inproceedings{Rabert2014ExpressionOA,
  title={Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector},
  author={Claudia Rabert and Ana Guti{\'e}rrez-Moraga and Alejandro Navarrete-Gallegos and Dar{\'i}o Navarrete-Campos and Le{\'o}n A Bravo and Manuel Gidekel},
  booktitle={International journal of molecular sciences},
  year={2014}
}
The current study isolated and characterized the Lip3F9 polypeptide sequence of Deschampsia antarctica Desv. (GeneBank Accession Number JX846628), which was found to be comprised of 291 base pairs and was, moreover, expressed in Pichia pastoris X-33 cells. The enzyme was secreted after 24 h of P. pastoris culture incubation and through induction with… CONTINUE READING