Expression of a Bacillus thuringiensisdelta-endotoxin cry1Ab gene in Bacillus subtilis and Bacillus licheniformis strains that naturally colonize the phylloplane of tomato plants (Lycopersicon esculentum, Mills).

@article{Theoduloz2003ExpressionOA,
  title={Expression of a Bacillus thuringiensisdelta-endotoxin cry1Ab gene in Bacillus subtilis and Bacillus licheniformis strains that naturally colonize the phylloplane of tomato plants (Lycopersicon esculentum, Mills).},
  author={Cristina Theoduloz and Andrea Reyes Vega and Marcela Mendes Salazar and Enrique Gonz{\'a}lez and Lu{\'i}s Meza-Basso},
  journal={Journal of applied microbiology},
  year={2003},
  volume={94 3},
  pages={375-81}
}
AIMS To introduce a cry gene into microorganisms that naturally colonize the phylloplane of tomato plants to improve the persistence of the Cry proteins for controlling a South American tomato moth (Tuta absoluta, Meyrick, 1917). METHODS AND RESULTS A cry1Ab gene isolated from a native Bacillus thuringiensis strain (LM-466), showing a relevant activity against T. absoluta larvae, was cloned into the shuttle vector pHT315 (Arantes and Lereclus 1991). The construct was introduced by… CONTINUE READING

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