Expression of a 48‐Kilodalton Growth‐Associated Protein in the Goldfish Retina

@article{PerroneBizzozero1987ExpressionOA,
  title={Expression of a 48‐Kilodalton Growth‐Associated Protein in the Goldfish Retina},
  author={N. Perrone-Bizzozero and L. Benowitz},
  journal={Journal of Neurochemistry},
  year={1987},
  volume={48}
}
Abstract: One of the most striking molecular correlates of optic nerve regeneration in the goldfish is the increased labeling of a 48 kilodalton (kD) acidic protein that is conveyed to the developing nerve endings from the retina by rapid axonal transport. The present study examined the biosynthesis and molecular characteristics of this protein. Retinas derived either from intact controls or from goldfish undergoing optic nerve regeneration (10–14 days postcrush) were pulse‐labeled with [3H… Expand
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References

SHOWING 1-10 OF 58 REFERENCES
Specific changes in rapidly transported proteins during regeneration of the goldfish optic nerve
TLDR
Two studies indicate a continuously changing pattern in the synthesis and/or degradation of proteins that are rapidly transported down the optic nerve during regeneration and point to molecular species potential significance in the establishment of the visual map upon the brain. Expand
Protein synthesis and transport in the regenerating goldfish visual system
TLDR
Analysis of proteins slowly transported to the optic tectum revealed changes following nerve crush similar to those observed in the retina, with enhanced labeling of both soluble and particulate tubulin and of 68–70K polypeptides. Expand
Increased transport of 44,000- to 49,000-dalton acidic proteins during regeneration of the goldfish optic nerve: a two-dimensional gel analysis
  • L. Benowitz, E. R. Lewis
  • Biology, Medicine
  • The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1983
TLDR
Proteins synthesized in goldfish retinal ganglion cells and rapidly transported to the terminals of regenerating optic nerves were analyzed by two-dimensional gel electrophoresis, indicating that the observed synthetic changes are accompanied by a net accumulation of the proteins. Expand
58,000 Dalton Intermediate Filament Proteins of Neuronal and Nonneuronal Origin in the Goldfish Visual Pathway
TLDR
A group of proteins in the goldfish optic nerve with a molecular weight of 58K daltons was analyzed by two‐dimensional gel electrophoresis and it was concluded that they are intermediate filament proteins. Expand
Changes in Protein Content of Goldfish Optic Nerve During Degeneration and Regeneration Following Nerve Crush
Abstract: After the goldfish optic nerve was crushed, the total amount of protein in the nerve decreased by about 45% within 1 week as the axons degenerated, began to recover between 2 and 5 weeks asExpand
In Vitro Protein Synthesis in the Goldfish Retinotectal Pathway During Regeneration: Evidence for Specific Axonal Proteins of Retinal Origin in the Optic Nerve
TLDR
In vitro incubations of retinas, optic nerves, or tecta in the presence of [35S]methionine indicate that proteins ON1 and ON2 are of retinal origin, consistent with the view that On1 and On2 are synthesized by retinal ganglion cells and are consequently of neuronal origin in the optic nerve. Expand
Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells
TLDR
The modulation of these proteins after axotomy is consistent with the possibility that they are involve in growth-specific functions and that the altered expression of a small number of genes is a crucial regulatory event in the transition of a mature neuron to a growth state. Expand
Biosynthesis and intra-axonal transport of proteins during neuronal regeneration.
TLDR
Overall, retinal ganglion cells show significant increases in the biosynthesis, in the intra-axonal transport, and in the glycosylation of proteins during regeneration, which are enhanced production of structural proteins, which persist throughout the regrowth process. Expand
A protein associated with axon growth, GAP-43, is widely distributed and developmentally regulated in rat CNS
TLDR
It is proposed that some step(s) in axon growth require production of GAP-43, and perhaps a small number of other “growth-associated proteins,” at rates much higher than those typical of mature neurons, and this hypothesis predicts that virtually all neurons synthesize G AP-43 at elevated levels during normal CNS development. Expand
Enhanced labeling of a retinal protein during regeneration of optic nerve in goldfish.
  • A. Heacock, B. Agranoff
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1976
TLDR
Goldfish retinas were examined for changes in the labeling pattern of protein during regeneration of retinal ganglion cell axons following unilateral optic nerve Crush and the protein fraction showing the increased incorporation of methionine in retina after optic nerve crush appears to be of the tubulin class. Expand
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