Expression of VSX1 in human corneal keratocytes during differentiation into myofibroblasts in response to wound healing.

@article{Barbaro2006ExpressionOV,
  title={Expression of VSX1 in human corneal keratocytes during differentiation into myofibroblasts in response to wound healing.},
  author={Vanessa Barbaro and Enzo Di Iorio and Stefano Ferrari and Luigi Bisceglia and Alessandro Ruzza and Michele De Luca and Graziella Pellegrini},
  journal={Investigative ophthalmology & visual science},
  year={2006},
  volume={47 12},
  pages={5243-50}
}
PURPOSE To characterize the expression of the visual system homeobox gene (VSX1) in human corneal keratocytes both in vitro and in vivo. METHODS The expression of VSX1 was evaluated through semiquantitative RT-PCR, immunofluorescence and in situ hybridization both in corneas (either freshly obtained or wounded) and in collagenase/hyaluronidase-isolated keratocytes grown in the absence or presence of serum to promote keratocyte-to-myofibroblast differentiation. RESULTS Quiescent or resting… CONTINUE READING
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