Expression of Streptococcus mutans gtf genes in Streptococcus milleri.

@article{Fukushima1992ExpressionOS,
  title={Expression of Streptococcus mutans gtf genes in Streptococcus milleri.},
  author={Kazuo Fukushima and Tadashi Ikeda and Howard K. Kuramitsu},
  journal={Infection and immunity},
  year={1992},
  volume={60 7},
  pages={2815-22}
}
The Streptococcus mutans glucosyltransferase (GTF) genes gtfB and gtfC were ligated into Escherichia coli-streptococcus shuttle plasmids and introduced into Streptococcus milleri. gtfB transformant KSB8 formed an S. mutans-like rough colony on mitis salivarius agar and expressed an extracellular GTF-I, of 158 kDa, and two cell-bound GTF-Is, of 158 and 135 kDa. gtfC transformant KSC43 formed a semirough colony on mitis salivarius agar and expressed primarily an extracellular GTF-SI, of 146 kDa… CONTINUE READING

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