Expression of Na(+)-independent amino acid transport in Xenopus laevis oocytes by injection of rabbit kidney cortex mRNA.

@article{Bertran1992ExpressionON,
  title={Expression of Na(+)-independent amino acid transport in Xenopus laevis oocytes by injection of rabbit kidney cortex mRNA.},
  author={Joan Bertran and Andreas Werner and Gertraud Stange and Daniel Markovich and Juerg Biber and Xavier Testar and Antonio Zorzano and Manuel Palac{\'i}n and Heini Murer},
  journal={The Biochemical journal},
  year={1992},
  volume={281 ( Pt 3)},
  pages={717-23}
}
Poly(A)+ mRNA was isolated from rabbit kidney cortex and injected into Xenopus laevis oocytes. Injection of mRNA resulted in a time- and dose-dependent increase in Na(+)-independent uptake of L-[3H]alanine and L-[3H]arginine. L-Alanine uptake was stimulated about 3-fold and L-arginine uptake was stimulated about 8-fold after injection of mRNA (25-50 ng, after 3-6 days) as compared with water-injected oocytes. T.I.C. of oocyte extracts suggested that the increased uptake actually represented an… CONTINUE READING

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