Expression of Haemonchus contortus pepsinogen in Caenorhabditis elegans.

  title={Expression of Haemonchus contortus pepsinogen in Caenorhabditis elegans.},
  author={Diane L Redmond and Caroline Clucas and I. Johnstone and David P. Knox},
  journal={Molecular and biochemical parasitology},
  volume={112 1},
A genomic copy of a gut-expressed Haemonchus contortus candidate vaccine antigen, pepsinogen, was isolated using the polymerase chain reaction (PCR). The isolated sequence was 4 kb in length and contained eight introns ranging in size from 54 to 1475 base pairs. This sequence, together with its 3' non-coding DNA region containing a polyadenylation signal sequence, was cloned into the Bluescript SK(+) vector immediately downstream of the Caenorhabditis elegans cpr-5 gene promoter. This promoter… CONTINUE READING


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