Expression of Glial Cell Line-Derived Neurotrophic Factor during Differentiation of Rat Bone Marrow Stromal Cells into Nerve Cells

@inproceedings{Ke2009ExpressionOG,
  title={Expression of Glial Cell Line-Derived Neurotrophic Factor during Differentiation of Rat Bone Marrow Stromal Cells into Nerve Cells},
  author={Deng Ke},
  year={2009}
}
Objective To investigate neural differentiation of rat bone marrow stromal cells(BMSCs) and expression of change of glial cell line-derived neurotrophic factor(GDNF).Methods BMSCs were harvested from rats and cultured in DMEM supplemented with 100 mL/L fetal bovine serum (FBS).They were characterized by flow cytometry of CD90 and CD45.At passage 4,BMSCs were induced by basic fibroblast growth factor(bFGF,10 μg/L),epidermal growth factor(EGF,10 μg/L) and both of them in DMEM with 100 mL/L FBS… CONTINUE READING

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