• Biology
  • Published 2002

Expression of Fusion Protein of HBV Core and PreS1 Gene in E. coli

@inproceedings{Xinchun2002ExpressionOF,
  title={Expression of Fusion Protein of HBV Core and PreS1 Gene in E. coli},
  author={Chen Xin-chun},
  year={2002}
}
Objective: To express the fusion protein of HBV core plus PreSl for development of HBV vaccine. Methods: pET-11d was used to express the fusion protein which was comprised of partial region (amino acids 3 - 55) of PreSl introduced at the C-terminal part of HBV core truncated protein (amino acids 1 - 155). The antigenicity of fusion protein was analysed by Western blot and EIA. The HBV core and PreSl gene was also cloned under the CMV promoter in eukaryotic expression vector. Results: Fusion… CONTINUE READING