Expression of Endogenous Thioredoxinh Gene and α-Amylase Activity in Transgenic Wheat with Antisense Thioredoxins Gene at Grain Filling Stage

@inproceedings{Jun2007ExpressionOE,
  title={Expression of Endogenous Thioredoxinh Gene and α-Amylase Activity in Transgenic Wheat with Antisense Thioredoxins Gene at Grain Filling Stage},
  author={Yin Jun},
  year={2007}
}
In order to clarify the mechanism of antisensethioredoxin s(anti-Trxs) gene fanction in transgenic wheat with pre-harvest sprouting resistance,the endogenous Trxh gene expression pattern and α-amylase activity in transgenic line 01TY70-14-3 and non-transgenic cultivar Yumai 70 were detected by RT-PCR and enzyme activity assay during seed filling stage.The results indicated that the mRNA transcript amounts of Trxh gene, Trxh and α-amylase activity in transgenic wheat seeds were lower distinctly… CONTINUE READING