Expression microarray analysis reveals genes associated with in vitro resistance to cisplatin in a cell line model

  title={Expression microarray analysis reveals genes associated with in vitro resistance to cisplatin in a cell line model},
  author={Mark Brian Watson and Michael J. Lind and Laura Smith and Philip J. Drew and Lynn Cawkwell},
  journal={Acta Oncologica},
  pages={651 - 658}
We aimed to investigate the mechanisms of cisplatin resistance using an in vitro cancer model. A derivative breast cancer cell line (MCF-7CR) was established which demonstrated significant resistance to cisplatin at clinically relevant low concentrations compared to the MCF-7 parental cell line. Expression microarray analysis was used to identify targets from a 3k cancer-related oligonucleotide platform which were differentially expressed between the derivative and parental cell lines. Real… 

Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?

It is suggested that the 26S proteasome may provide a general predictive biomarker for radiotherapy outcome and be associated with radioresistant laryngeal tumors in a small pilot immunohistochemical study.

Oestrogen receptor-regulated glutathione S-transferase mu 3 expression attenuates hydrogen peroxide-induced cytotoxicity, which confers tamoxifen resistance on breast cancer cells

Evidence is provided that glutathione S-transferase mu 3 may play an important role in preventing breast cancer treatment-induced cellular cytotoxicity and in suppressing tumourigenic ability of MCF-7 cells and increase tumour cell apoptosis.

Establishment of a predictive genetic model for estimating chemotherapy sensitivity of colorectal cancer with synchronous liver metastasis.

The predictive model involving the seven genes listed had high accuracy in estimating chemotherapy sensitivity to the FOLFOX4 regimen, with an overall accurate predication rate of 93.3%.

The gene expression profiles of medulloblastoma cell lines resistant to preactivated cyclophosphamide.

TAP1, a protein belonging to the ABC transporter family is normally involved in major histocompatibility class I (MHC I) antigen processing, suggests the possible role of multidrug resistance (MDR), albeit atypical (which means it does not involve the usual MDR1 and MRP glycoproteins), in medulloblastoma's CP-resistance.

SCG3 Transcript in Peripheral Blood Is a Prognostic Biomarker for REST-Deficient Small Cell Lung Cancer

In peripheral blood of lung cancer patients and cancer-free individuals, it was found that SCG3 was more tumor-specific and more sensitive than other chromogranin transcripts as a biomarker of circulating tumor cells.

A plausible role for actin gamma smooth muscle 2 (ACTG2) in small intestinal neuroendocrine tumorigenesis

ACTG2 is expressed in a fraction of SI-NETs, can inhibit cell growth in vitro, and is positively regulated by miR-145, and theoretical therapeutic strategies based on these results are discussed.

SCG 3 Transcript in Peripheral Blood Is a Prognostic Biomarker for REST-Deficient Small Cell Lung Cancer

It is found that SCG3 mRNA, a component of the REST-dependent neurosecretory transcriptional profile, provides a sensitive prognostic biomarker for noninvasive monitoring of neuroendocrine lung cancer.

Dual-specificity phosphatases: therapeutic targets in cancer therapy resistance

Emerging evidence indicates that the DUSP family is aberrantly expressed in human malignancies and plays critical roles in determining sensitivity to a wide range of cancer therapeutic strategies through regulation of the MAPK signaling pathways.



Induction of MDR1-gene expression by antineoplastic agents in ovarian cancer cell lines.

The data indicate that mdr1-induction by antineoplastics is one of the reasons for failure of ovarian cancer therapy but may vary from one individual to another.

Establishment of cisplatin-resistant variants of human neuroblastoma cell lines, TGW and GOTO, and their drug cross-resistance profiles

These CDDP-resistant variants and clones derived from two neuroblastoma cell lines by exposure to low doses of cisplatin may be useful for studying the mechanisms of low-level drug resistance in neuroblastomas.

Expression of human mu or alpha class glutathione S-transferases in stably transfected human MCF-7 breast cancer cells: effect on cellular sensitivity to cytotoxic agents.

It is concluded that, at the levels of GST obtained in this transfection model system, overexpression of GST mu or GST alpha 2 is not by itself sufficient to confer resistance to these anticancer agents, and these studies do not exclude the possibility that GST may be a marker of drug resistance or that other gene products not expressed in MCF-7 cells might cooperate with GST to confer drug resistance.

Prolactin acts as a potent survival factor for human breast cancer cell lines

It is demonstrated that prolactin protects human breast cancer cell lines against apoptosis and this may have important implications for cancer treatment.

Expression of human glutathione S-transferases in Saccharomyces cerevisiae confers resistance to the anticancer drugs adriamycin and chlorambucil.

Examining human Alpha-class and Pi-class GST cDNAs that encode GST B1B1 and GST pi in the yeast Saccharomyces cerevisiae provides direct evidence that the over-expression of GST in cells can confer resistance to anticancer drugs.

Prolactin Overexpression by MDA-MB-435 Human Breast Cancer Cells Accelerates Tumor Growth

Data support a role for breast PRL as a growth/anti-apoptotic factor and suggest that it may serve as a novel therapeutic target for the treatment of breast cancer.

Updates on functions of ceramide in chemotherapy-induced cell death and in multidrug resistance.

  • B. OgretmenY. Hannun
  • Biology, Chemistry
    Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy
  • 2001
The sphingolipid ceramide, a bioeffector lipid, is known to regulate anti-proliferative responses, such as apoptosis, growth arrest, differentiation and senescence in various human cancer cell lines.

Platinum compounds in the treatment of advanced breast cancer.

  • M. Martín
  • Biology, Chemistry
    Clinical breast cancer
  • 2001
Cisplatin, carboplatin, and perhaps, oxaliplatin appear to have some antitumor activity in MBC and can be combined safely with other agents that are active in this disease.