Expression levels of human P-glycoprotein in in vitro cell lines: correlation between mRNA and protein levels for P-glycoprotein expressed in cells.

@article{Shirasaka2009ExpressionLO,
  title={Expression levels of human P-glycoprotein in in vitro cell lines: correlation between mRNA and protein levels for P-glycoprotein expressed in cells.},
  author={Yoshiyuki Shirasaka and Reiko Konishi and Nana Funami and Yuko Kadowaki and Yuri Nagai and Toshiyuki Sakaeda and Shinji Yamashita},
  journal={Biopharmaceutics & drug disposition},
  year={2009},
  volume={30 3},
  pages={149-52}
}
The purpose of this study was to investigate the correlation between mRNA and protein levels for P-glycoprotein (P-gp) expressed in various cell lines to validate the estimation of P-gp activity from its mRNA levels. P-gp expression levels in various cell monolayers, normal, P-gp-induced, P-gp-highly induced, (multidrug resistance, MDR) MDR1-knockdown (A2-2) and MDR1-knockdown (B2-2) Caco-2 cells and MDCKII/MDR1 cells, were quantified by real-time quantitative polymerase chain reaction (PCR… CONTINUE READING

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P - gp expression levels in various cell monolayers , normal , P - gp - induced , P - gp - highly induced , ( multidrug resistance , MDR ) MDR1-knockdown ( A2 - 2 ) and MDR1-knockdown ( B2 - 2 ) Caco-2 cells and MDCKII / MDR1 cells , were quantified by real - time quantitative polymerase chain reaction ( PCR ) and western blot analysis .
P - gp expression levels in various cell monolayers , normal , P - gp - induced , P - gp - highly induced , ( multidrug resistance , MDR ) MDR1-knockdown ( A2 - 2 ) and MDR1-knockdown ( B2 - 2 ) Caco-2 cells and MDCKII / MDR1 cells , were quantified by real - time quantitative polymerase chain reaction ( PCR ) and western blot analysis .
P - gp expression levels in various cell monolayers , normal , P - gp - induced , P - gp - highly induced , ( multidrug resistance , MDR ) MDR1-knockdown ( A2 - 2 ) and MDR1-knockdown ( B2 - 2 ) Caco-2 cells and MDCKII / MDR1 cells , were quantified by real - time quantitative polymerase chain reaction ( PCR ) and western blot analysis .
P - gp expression levels in various cell monolayers , normal , P - gp - induced , P - gp - highly induced , ( multidrug resistance , MDR ) MDR1-knockdown ( A2 - 2 ) and MDR1-knockdown ( B2 - 2 ) Caco-2 cells and MDCKII / MDR1 cells , were quantified by real - time quantitative polymerase chain reaction ( PCR ) and western blot analysis .
P - gp expression levels in various cell monolayers , normal , P - gp - induced , P - gp - highly induced , ( multidrug resistance , MDR ) MDR1-knockdown ( A2 - 2 ) and MDR1-knockdown ( B2 - 2 ) Caco-2 cells and MDCKII / MDR1 cells , were quantified by real - time quantitative polymerase chain reaction ( PCR ) and western blot analysis .
P - gp expression levels in various cell monolayers , normal , P - gp - induced , P - gp - highly induced , ( multidrug resistance , MDR ) MDR1-knockdown ( A2 - 2 ) and MDR1-knockdown ( B2 - 2 ) Caco-2 cells and MDCKII / MDR1 cells , were quantified by real - time quantitative polymerase chain reaction ( PCR ) and western blot analysis .
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