Expression levels of drug-metabolizing enzyme, transporter, and nuclear receptor mRNAs in a novel three-dimensional culture system for human hepatocytes using micro-space plates.

@article{Nishimura2011ExpressionLO,
  title={Expression levels of drug-metabolizing enzyme, transporter, and nuclear receptor mRNAs in a novel three-dimensional culture system for human hepatocytes using micro-space plates.},
  author={Masuhiro Nishimura and Yoko Ejiri and Sanae Kishimoto and Satoshi Suzuki and Tetsuo Satoh and Toru Horie and Shizuo Narimatsu and Shinsaku Naito},
  journal={Drug metabolism and pharmacokinetics},
  year={2011},
  volume={26 2},
  pages={
          137-44
        }
}
We evaluated a novel three-dimensional primary culture system using micro-space plates to determine the expression levels of 61 target (drug-metabolizing enzymes, transporters, and nuclear receptors) mRNAs in human hepatocytes. We measured mRNA expression levels of many target genes in four lots of cryopreserved human hepatocyte primary cells after 120 h of culture and compared differences in mRNA expression levels between cultures using traditional plates and those using micro-space plates. In… 

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