Expression level of Ubc9 protein in rat tissues.

  title={Expression level of Ubc9 protein in rat tissues.},
  author={Filip Gołebiowski and Aneta Szulc and Monika Sakowicz and Andrzej Szutowicz and Tadeusz Pawełczyk},
  journal={Acta biochimica Polonica},
  volume={50 4},
Ubc9 is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. In this report we describe a simple and efficient method for obtaining pure human recombinant Ubc9 protein. The purified Ubc9 retained its native structure and was fully active in an in vitro sumoylation assay with the promyelocytic leukaemia (PML) peptide as a substrate. In order to better understand the physiology of Ubc9 protein we examined its levels… CONTINUE READING
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