Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells

@inproceedings{Tsakiridis2009ExpressionindependentGT,
  title={Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells},
  author={Anestis Tsakiridis and Elena Tzouanacou and Afifah Rahman and Douglas Colby and Richard A. Axton and Ian Chambers and Valerie Wilson and Lesley M Forrester and Joshua M Brickman},
  booktitle={Nucleic acids research},
  year={2009}
}
Promoterless gene trap vectors have been widely used for high-efficiency gene targeting and random mutagenesis in embryonic stem (ES) cells. Unfortunately, such vectors are only effective for genes expressed in ES cells and this has prompted the development of expression-independent vectors. These polyadenylation (poly A) trap vectors employ a splice donor to capture an endogenous gene's polyadenylation sequence and provide transcript stability. However, the spectrum of mutations generated by… CONTINUE READING