Expression in Pichia pastoris and purification of Aspergillus awamori glucoamylase catalytic domain.

@article{Heimo1997ExpressionIP,
  title={Expression in Pichia pastoris and purification of Aspergillus awamori glucoamylase catalytic domain.},
  author={Heikki Heimo and Kaisa Palmu and Ilpo Suominen},
  journal={Protein expression and purification},
  year={1997},
  volume={10 1},
  pages={70-9}
}
In this paper we report the expression in Pichia pastoris, purification, and characterization of the Aspergillus awamori glucoamylase catalytical domain (GAc). Pichia pastoris produced GAc to the level of 0.4 g per liter medium. This production level is about the same level as that gained for recombinant GA from Aspergillus and about 100-fold more than previously achieved by Saccharomyces cerevisiae. The GAc expressed in Pichia pastoris was purified by two independent chromatographic methods… CONTINUE READING
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