Expression in Escherichia coli: purification and properties of the recombinant human general transcription factor rTFIIB.

@article{Moncollin1992ExpressionIE,
  title={Expression in Escherichia coli: purification and properties of the recombinant human general transcription factor rTFIIB.},
  author={Vincent Moncollin and Laurent Schaeffer and Christian Chalut and Jean Marc Egly},
  journal={Protein expression and purification},
  year={1992},
  volume={3 5},
  pages={374-9}
}
The human class II transcription factor TFIIB (rTFIIB) was overexpressed in Escherichia coli using a T7 RNA polymerase expression system and further purified to apparent homogeneity. The purified rTFIIB is identical to the endogenous factor according to the following criteria: molecular weight, microsequencing and mass spectra studies, ability to recognize the stable preinitiation complex formed between TFIID and the adenovirus 2 major late TATA box as demonstrated by gel shift as well as by… CONTINUE READING

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